abc method vectastain abc kit (Vector Laboratories)

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Vector Laboratories abc method vectastain abc kit
Abc Method Vectastain Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 426 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abc+method+vectastain+abc+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Goat+IgG)/pmc12937272-61-8-14
Average 94 stars, based on 426 article reviews

abc method vectastain abc kit - by Bioz Stars, 2026-09

94/100 stars

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Immunohistochemistry:

Article Title: Regulation of 4-HNE via SMARCA4 Is Associated with Worse Clinical Outcomes in Hepatocellular Carcinoma
Article Snippet: .. IHC of 4-HNE, GPX4, FSP1, GCH1, and SMARCA4 was performed using the avidin and biotinylated enzyme (ABC) method (VECTASTAIN ABC kit; Vector Laboratories, Newark, CA, USA) or the polymer method (ImmPRESS Reagent kit; Vector Laboratories), and the staining was visualized using Vector 3,3′-Diaminobenzidine Substrate (Vector Laboratories). ..

Article Title: Regulation of 4-HNE via SMARCA4 Is Associated with Worse Clinical Outcomes in Hepatocellular Carcinoma.
Article Snippet: .. IHC of 4-HNE, GPX4, FSP1, GCH1, and SMARCA4 was performed using the Biomedicines 2023, 11, 2278 3 of 15 avidin and biotinylated enzyme (ABC) method (VECTASTAIN ABC kit; Vector Laboratories, Newark, CA, USA) or the polymer method (ImmPRESS Reagent kit; Vector Laboratories), and the staining was visualized using Vector 3,3′-Diaminobenzidine Substrate (Vector Laboratories). ..

Article Title: The Small Heat Shock Protein HSP25/27 (HspB1) Is Abundant in Cultured Astrocytes and Associated with Astrocytic Pathology in Progressive Supranuclear Palsy and Corticobasal Degeneration
Article Snippet: .. Following antigen retrieval using Vector unmasking solution formalin-fixed brain sections were analyzed by immunohistochemistry as described previously [ ], using the avidin-biotin complex (ABC) method (Vectastain ABC kit, Vector Laboratories, Burlingame, CA). .. For double immunolabeling, sections were blocked in 5% ChemiBLOCKER (Chemicon, Millipore, Billerica, MA, USA) and 2% donkey serum in 0.1 M Tris, pH 7.6 supplemented with 0.1% Triton X-100 (TrisT) overnight at room temperature after antigen retrieval.

Avidin-Biotin Assay:

Article Title: Isolation and characterization of Helicobacter suis from human stomach
Article Snippet: For primary antibody conjugation, the section was blocked with serum and incubated overnight with anti-TFF2 antibody (1:100) [a kind gift from Sir Nicholas Wright ( )] or anti-TFF3 antibody (1:1,000) (UCLNT3, Thermo Fisher Scientific). .. The avidin-biotin complex (ABC) method (Vectastain ABC kit, Vector) was used to visualize staining. .. The Peroxidase–DAB system was used for TFF3 staining, and the Alkaline phosphatase–Vector Red system was used for TFF2 staining.

Article Title: A Preliminary Study on the Role of Orexin A in Leydig Cell Steroidogenesis and Its Implications for Fertility in Alpacas (Vicugna pacos)
Article Snippet: .. Immunostaining was carried out using the avidin-biotin-peroxidase complex (ABC) method (Vectastain ABC Kit, PK-6105, Vector Laboratories, Burlingame, CA, USA). .. Sections were incubated overnight at 4 ◦C with primary antibodies diluted in PBS-BSA 1% under the following conditions: mouse anti-OxA monoclonal antibody (MAB763, R&D Systems) at a 1:150 dilution and rabbit anti-OX1R polyclonal antibody (ab3092, Millipore) at a 1:200 dilution.

Article Title: Superficial angiomyxoma in a pregnant cow
Article Snippet: Additional sections of the mass were also stained with alcian blue at pH 2.5 and subjected to immunohistochemistry using primary antibodies directed against vimentin (1 in 100 dilution, V9, mouse monoclonal; DAKO), α-smooth muscle actin (α-SMA; 1 in 400 dilution, 1A4, mouse monoclonal; DAKO), desmin (1 in 50 dilution, D33, mouse monoclonal; DAKO), and von Willebrand factor (vWF; 1 in 400 dilution, rabbit polyclonal; DAKO), according to a previously-described technique (Romanucci et al. ). .. Immune complexes were treated with secondary biotinylated goat anti-mouse or anti-rabbit antibody (1 in 200 dilution; Vector Laboratories, Inc., Burlingame, CA) and subsequently detected using an avidin-biotin complex (ABC) method (Vectastain® ABC Kit; Vector Laboratories, Inc.). .. Peroxidase activity was detected using 0.1% hydrogen peroxide in 3,3′-diaminobenzidine solution (Sigma–Aldrich, Saint Louis, MO).

Article Title: A Preliminary Study on the Role of Orexin A in Leydig Cell Steroidogenesis and Its Implications for Fertility in Alpacas ( Vicugna pacos )
Article Snippet: .. Immunostaining was carried out using the avidin-biotin-peroxidase complex (ABC) method (Vectastain ABC Kit, PK-6105, Vector Laboratories, Burlingame, CA, USA). .. Sections were incubated overnight at 4 °C with primary antibodies diluted in PBS-BSA 1% under the following conditions: mouse anti-OxA monoclonal antibody (MAB763, R&D Systems) at a 1:150 dilution and rabbit anti-OX1R polyclonal antibody (ab3092, Millipore) at a 1:200 dilution.

Article Title: Microvascular, Biochemical, and Clinical Impact of Hyperbaric Oxygen Therapy in Recalcitrant Diabetic Foot Ulcers
Article Snippet: Then, anti-rabbit secondary antibody (1:200) (Santa Cruz Biotechnology, Dallas, TX, USA) was applied for 30 min. .. The avidin biotin complex (ABC) method (Vectastain ABC kit, Vector, Burlingame, CA, USA) was used according to the manufacturer’s instructions. .. The antigen–antibody reaction was developed using diaminobenzidine (DAB) (DAB substrate kit, Abcam, Cambridge, UK) as a peroxidase substrate, rendering CD31-positive cells with a brown staining.

Polymer:

Staining:

Immunostaining:

Article Title: A Preliminary Study on the Role of Orexin A in Leydig Cell Steroidogenesis and Its Implications for Fertility in Alpacas ( Vicugna pacos )
Article Snippet: .. Immunostaining was carried out using the avidin-biotin-peroxidase complex (ABC) method (Vectastain ABC Kit, PK-6105, Vector Laboratories, Burlingame, CA, USA). .. Sections were incubated overnight at 4 °C with primary antibodies diluted in PBS-BSA 1% under the following conditions: mouse anti-OxA monoclonal antibody (MAB763, R&D Systems) at a 1:150 dilution and rabbit anti-OX1R polyclonal antibody (ab3092, Millipore) at a 1:200 dilution.

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